Observations on Molecular Weight Determinations on Polyacrylamide Gel
نویسندگان
چکیده
منابع مشابه
Observations on molecular weight determinations on polyacrylamide gel.
1. A simple internal calibration technique was used to construct accurate molecular weight-mobility prof?les on polyacrylamide gels with well characterized proteins, and the existence of a “critical point” was observed for small polypeptides. The useful size range limits were determined for several gel concentrations. Apparent molecular weights generally fell within 5 to 6% of literature values...
متن کاملThe reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
Forty proteins with polypeptide chains of well characterized molecular weights have been studied by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate following the procedure of Shapiro, Vifiuela, and Maize1 (Biochem. Biophys. Res. Commun., 28, 815 (1967)). When the electrophoretic mobilities were plotted against the logarithm of the known polypeptide chain molecular w...
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A new technique was devised to be used with molecular weight determinations of proteins on polyacrylamide gels. Internal standards consisting of proteins covalently linked to l-dimethylaminonaphthalene-S-sulfonyl(DANS-) groups were prepared. DANSderivatives of insulin and monomer, dimer, trimer, tetramer, pentamer, and hexamer of egg white lysozyme and serum albumin covered molecular weights fr...
متن کاملDiscontinuous electrophoresis on polyacrylamide gel slab.
D ISCOT1XUOUS ELECT1IO1’HORESIS in polyacrylamide rods has been used for separation of serum proteins (1). In spite of satisfactory resolution in tile rod, the technic renhains cumbersome, and comparison of the closely migrating cOnlpoflents inaccurate. Akroyd (2) reported discontinuous electrophoresis On polyacrylamide gel slab, which alleviates many of the difficulties of the rod. We llave co...
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The molecular weight (MW) of Sindbis virion ribonucleic acid (RNA) determined by gel electrophoresis was in a range of 3.89 x 10(6) to 4.45 x 10(6) daltons. Upon denaturation with urea, it separated into fragments having an MW of 1.76 x 10(6) daltons. Viral-specific Sindbis 26S RNA contained a major species having an MW of 1.76 x 10(6) daltons.
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1969
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)94310-3